Proteoglycans synthesized by cultured mouse osteoblasts.
نویسندگان
چکیده
منابع مشابه
Isolation and characterization of proteoglycans synthesized by cultured mesangial cells.
Rat mesangial cells selected by long-term culture of glomeruli exhibited a hill and valley appearance in the confluent state and were stained with antibodies against vimentin and desmin, suggesting that they are smooth muscle-like mesangial cells. The glycoconjugates produced by the cells were metabolically labeled with [35S]sulfate and [3H]glucosamine and extracted with 4 M guanidine HCl conta...
متن کاملIsolation and characterization of proteoglycans synthesized by mouse osteoblastic cells in culture during the mineralization process.
Proteoglycans in mineralized (0.5 M-EDTA/4 M-guanidinium chloride-extractable) and non-mineralized (4 M-guanidinium chloride-extractable) matrices synthesized by a mouse osteoblastic-cell line MC3T3-E1 were characterized at different phases of mineralization in vitro. Cell cultures were labelled with [35S]sulphate and either [3H]glucosamine or 3H-labelled amino acids. At the mineralization phas...
متن کاملIsolation and preliminary characterization of proteoglycans synthesized by skeletal muscle.
The proteoglycans synthesized by cartilage both in vivo and in vitro have been well characterized and serve as a standard for comparison with proteoglycans from other tissues. Both cartilage and muscle are mesenchymally derived tissues; yet proteoglycans synthesized by skeletal muscle have not been studied. This report describes the isolation and preliminary characterization of skeletal muscle ...
متن کاملChanges in the Sulfated Proteoglycans Synthesized by “Aging” Chondrocytes
Sulfated proteoglycans synthesized by intact embryonic chick vertebral cartilages maintained in organ culture were characterized by labeling the vertebral chondrocytes with [35S]sulfate. More than 95% of the sulfated macromolecules synthesized by the cartilages are retained in their extracellular matrix. Sucrose velocity sedimentation gradient analyses revealed that the intact cartilages synthe...
متن کامل[Hydroxyapatite-binding phosphoproteins synthesized by osteoblasts and fibroblasts].
The present study attempted to explore the newly synthesized phosphoproteins secreted by osteoblastlike cells and fibroblasts from rat pup calvaria and skin. The phosphoproteins, which are non-collagenous proteins, are posttranslationally phosphorylated by the kinases and secreted into the extracellular matrix and medium in the osteoblast-like cell and fibroblast cultures. The osteoblast-enrich...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1987
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)61194-9